Trinocular Inverted LED Fluorescence Microscope | 4-Channel B/G/U/V

An inverted LED fluorescence microscope is primarily used for multi-fluorophore live cell imaging, tissue culture observation, and immunofluorescence imaging. The inverted design allows researchers to observe living cells submerged in media inside thick culture flasks or petri dishes.

It means the microscope is equipped with a 4-position filter cube containing Blue (B), Green (G), Violet (V/U), and Ultraviolet (UV) excitation filters. This allows the microscope to excite a massive range of fluorophores sequentially without changing hardware.

A B/G model only has two filters, typically limiting you to GFP/FITC (Blue excitation) and RFP/TRITC (Green excitation). A B/G/UV model adds the critical Ultraviolet and Violet channels, enabling you to simultaneously perform nuclear staining (like DAPI) in the same assay.

DAPI is a fluorescent stain that binds strongly to DNA, making it ideal for nuclear staining. To view DAPI, you must use the UV excitation filter (330-385nm range), which is included in this 4-channel microscope model.

The UV filter excites DAPI and Hoechst. The Blue filter excites FITC, GFP, Cy2, and Alexa Fluor 488. The Green filter excites TRITC, RFP, Cy3, and Texas Red. This provides unparalleled versatility for multi-channel cellular imaging.

The LED inverted fluorescence microscope with 4-position filter cube for DAPI FITC TRITC and GFP allows researchers to conduct complex multi-protein viability assays and apoptosis studies that require marking the cell nucleus (DAPI) alongside two other proteins.

Our inverted trinocular fluorescence microscope with UV 330-385nm blue 460-490nm green 510-550nm filter provides the exact optimal UV excitation band required to make nuclear stains fluoresce brilliantly blue.

Long Working Distance (LWD) objectives provide a greater physical gap between the lens and the specimen. This is critical for inverted microscopes because it allows you to focus through the thick plastic bottoms of cell culture flasks without hitting the vessel.

You image them sequentially. You switch to the UV filter for DAPI, then the Blue filter for FITC, then the Green filter for TRITC. Using camera software, these three images are seamlessly overlaid into a single, multi-color composite image.

LEDs provide instant on/off capabilities, incredibly long lifespans (>65,000 hrs), zero toxic mercury disposal hazards, and produce very little heat. The inverted fluorescence microscope LED long life with brightness control for sensitive specimens prevents photo-bleaching and thermal damage to live cells.

Upright microscopes view samples from above (ideal for prepared, dead samples on glass slides). Inverted microscopes view from below, providing the necessary working distance to observe living, growing cells submerged in liquid media inside thick petri dishes and flasks.

They are the standard for advanced tissue culture labs, IVF embryology centers, cancer and oncology research facilities, molecular biology institutes, and pharmaceutical drug discovery labs.

The inverted fluorescence trinocular B/G/UV LED price India varies based on the digital camera attachments you require. Please request a quote for competitive institutional pricing from GBS Microscopes.

Yes, the trinocular inverted fluorescence microscope with C-mount camera port for digital imaging allows you to easily attach high-sensitivity CMOS cameras to capture faint fluorescent emissions. The 100% light split ensures max photons hit the camera sensor.

Immunofluorescence is a technique that uses fluorescent-labeled antibodies to detect specific target proteins in a cell. Common dyes like Alexa Fluor 488 and 594 require the Blue and Green filters respectively, making this 4-channel model perfectly suited for the task.